Part Number
Part Number:
NSN:
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Item Name:
TNF-ALPHA ELISA KIT
CAGE Information
Code | Company |
---|---|
4ZTH3 | ASSAYPRO LLC |
Characteristics (Decoded)
MRC | Requirements Statement | Clear Text Reply |
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CXCY | PART NAME ASSIGNED BY CONTROLLING AGENCY | ASSYMAX HUMAN TUMOR NECROSIS FACTOR ALPHA (TNF-ALPHA) ELISA KIT |
FEAT | SPECIAL FEATURES | THE ASSAYMAX HUMAN TNF-ALPHA ELISA KIT IS DESIGNED FOR DETECTION OF TNF-ALPHA IN HUMAN PLAMSA, SERUM OR CELL CULTURE SUPERNATANTS. THIS ASSAY EMPLOYS A QUANTITATIVE SANDWICH ENZYME IMMUNOASSAY TECHNIQUE THAT MEASURES TNF-ALPHA IN 4.5 HOURS. A MURINE MONOCLONAL ANTIBODYSPECIFIC FOR HUMAN TNF-ALPHA HAS BEEN PRE-COATED ONTO A MICROPLATE. TNF-ALPHA IN STANDARDS AND SAMPLES IS SANDWICHED BY THE IMMOBILIZED ANTIBODY AND A BIOTINYLATED POLYCLONAL ANTIBODY SPECIFIC FOR HUMAN TNF-ALPHA, WHICH IS RECOGNIZED BY A STREPTAVIDIN-PEROXIDASE CONJUGATE. ALL UNBOUND MATERAL IS THEN WASHED AWAY AND A PEROXIDASE ENZYME SUBSTRATE IS ADDED. THE COLOR DEVELOPMENT IS STOPPED AND THE INTENSITY OF THE COLOR IS MEASURED. |